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Updated: May 17, 2026

Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
Published on: October 29, 2010
Evaluation of electron crystallographic data from images of two-dimensional crystals
1Department of Molecular Biosciences, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
Abstract:
In the past, various formats have been used to present data fidelity of electron crystallographic data from images of two-dimensional crystals. While each scheme provides some information about the data, not all schemes are equally useful for presenting data reliability. This brief chapter gives guidelines on how to look at datasets that are solely derived from images, and on how to summarize the data structure through graphical outputs that provide unbiased and detailed information about data quality. By the time of publication, the actual procedures described here will have been integrated into the 2dx package and therefore, these evaluation tools will be available at a "push of a button." Because of this, the chapter focuses on brief explanations for why certain ways of presenting data make more sense than others.
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