[Expression of HIF-1α and VEGF in human dental pulp cells under mechanical stretch]
Fu-lan Wei1, Jie Geng, Chun-ling Wang
1Department of Orthodontics, Shandong University, Shandong Province, China. weifl@sdu.edu.cn
Purpose:
To examine the expression of HIF-1α and VEGF after application of mechanical stretch on human dental pulp cells (HDPCs), and to investigate the role of them in maintaining homeostasis of dental pulp during orthodontic movement.
Methods:
HDPCs were subjected to 15% elongation by 1.0 Hz stretching frequency for 6, 12, 24, 48 and 72 h. The expression of HIF-1α and VEGF mRNA was measured by real-time polymerase chain reaction (PCR). SPSS12.0 software package was applied for statistical analysis.
Results:
At the initial time point, HIF-1α mRNA had a weak expression. The mRNA level of HIF-1α increased gradually and then decreased towards its pre-pressure levels. The mRNA level of VEGF was upregulated in a time-dependent manner.
Conclusions:
The mRNA expression of HIF-1α and VEGF was enhanced by mechanical stress in HDPCs, which indicates that HIF-1α and VEGF may play an important role in retaining homeostasis of dental pulp during orthodontic movement.
