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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: May 17, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Rice artificial hybridization for genetic analysis.

Xueyan Sha1

  • 1Rice Research Station, Louisiana State University Agricultural Center, Crowley, LA, USA. xsha@agcenter.lsu.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2012
PubMed
Summary

Artificial hybridization is key for rice genetic improvement, creating new variations through controlled crosses. This process involves careful parent selection, emasculation, and pollination for successful gene transfer.

Area of Science:

  • Plant Science
  • Genetics
  • Agricultural Science

Background:

  • Artificial hybridization has ancient roots but was scientifically established with Gregor Mendel's genetic studies.
  • It remains the primary method for transferring genes in self-pollinated rice, creating genetic variation via recombination.

Purpose of the Study:

  • To detail the process and requirements for successful artificial hybridization in rice.
  • To highlight the importance of gene recombination for novel genetic variation in rice breeding.

Main Methods:

  • Selection of suitable panicles from female and male parent plants.
  • Emasculation of female parents using methods like hot water or vacuum treatments.
  • Pollination of emasculated flowers with pollen from selected male parents.

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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

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Last Updated: May 17, 2026

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Main Results:

  • Hot water and vacuum emasculation are identified as robust and reliable techniques.
  • Successful hybridization depends on understanding parental germplasm and rice reproductive biology.

Conclusions:

  • Artificial hybridization is crucial for generating genetic diversity in rice.
  • Efficient rice breeding programs require knowledge of plant reproductive biology and synchronized flowering techniques.