Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
Taping Over Different Ground Profiles01:12

Taping Over Different Ground Profiles

Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KAS-CUT&Tag for direct mapping of transcription bubbles.

bioRxiv : the preprint server for biology·2026
Same author

Promoting Trust and Engagement Through Health Literacy Education: Enhancing the Therapeutic Alliance with People Hospitalized for Acute Psychiatric Care.

Issues in mental health nursing·2026
Same author

Layer-specific genetic variation unlocks secondary metabolite diversity in long-lived clonal peppermint.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Superabundant microRNAs are transcribed from human rDNA spacer promoters insulated by CTCF.

Science advances·2026
Same author

RNA polymerase II: the elephant in the room.

Trends in genetics : TIG·2026
Same author

Cell-cycle-dependent repression of histone gene transcription by histone H4.

Nature structural & molecular biology·2026

Related Experiment Video

Updated: May 17, 2026

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)
09:00

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)

Published on: January 28, 2020

TILLING and ecotilling for rice.

Jennifer L Cooper1, Steven Henikoff, Luca Comai

  • 1Department of Chemistry, University of Akron, Akron, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2012
PubMed
Summary

This study presents a high-throughput protocol for Targeting Induced Local Lesions in Genomes (TILLING) in rice. This method efficiently identifies mutations for crop improvement and genetic diversity studies.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Agricultural science

Background:

  • Mutagenesis is crucial for understanding gene function and enhancing crop traits.
  • Targeting Induced Local Lesions in Genomes (TILLING) is a reverse genetic approach for identifying induced point mutations.
  • The TILLING strategy has been successfully adapted across various plant and animal species.

Purpose of the Study:

  • To describe a high-throughput protocol for TILLING in rice.
  • To facilitate gene function studies and crop improvement in rice.
  • To adapt TILLING methods for cataloguing natural genetic variation (Ecotilling).

Main Methods:

  • Fluorescently tagged primers amplify gene segments in rice.
  • Denaturation and reannealing create heteroduplexes between mutated and wild-type sequences.

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

Related Experiment Videos

Last Updated: May 17, 2026

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)
09:00

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)

Published on: January 28, 2020

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

  • Single-strand-specific nucleases cleave heteroduplexes, followed by analysis on denaturing polyacrylamide gels using the LI-COR DNA analyzer.

    • Main Results:

    • A robust protocol for high-throughput TILLING in rice has been established.
    • Several rice TILLING populations are available, with a public mutation screening service offered.
    • The TILLING methodology is adaptable for Ecotilling to study natural nucleotide variation.

    Conclusions:

    • The developed high-throughput TILLING protocol is effective for rice research.
    • This method supports both targeted mutation discovery and the study of natural genetic diversity.
    • The availability of rice TILLING populations and screening services advances plant genetics research.