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Related Experiment Video

Updated: May 17, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Rice proteomic analysis: sample preparation for protein identification.

Ganesh Kumar Agrawal1, Nam-Soo Jwa, Young-Ho Jung

  • 1Research Laboratory for Biotechnology and Biochemistry, Kathmandu, Nepal.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2012
PubMed
Summary
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This study details rice proteomics protocols, focusing on protein isolation, separation, and identification using mass spectrometry. These methods advance our understanding of rice biology and cultivation.

Area of Science:

  • Proteomics
  • Plant Science
  • Biochemistry

Background:

  • Rice is a globally significant food and cereal crop.
  • Rice proteomics research has advanced significantly since the 1990s.
  • Global proteomics studies in rice have expanded since 2000, examining various growth and environmental conditions.

Purpose of the Study:

  • To provide a detailed overview of established protocols for rice proteomics.
  • To describe methods for protein isolation, separation, detection, and identification in rice.
  • To highlight the utility of gel-based proteomics and mass spectrometry for rice research.

Main Methods:

  • Protein isolation from diverse rice tissues, organs, and organelles.
  • Two-dimensional (2-D) gel electrophoresis for protein separation.

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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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Last Updated: May 17, 2026

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  • Mass spectrometry for protein identification.

    • Main Results:

    • Established protocols enable comprehensive analysis of the rice proteome.
    • Gel-based proteomics coupled with mass spectrometry is a robust platform for rice samples.
    • Detailed methodologies facilitate further research in rice biology and crop improvement.

    Conclusions:

    • The described protocols provide a foundation for advanced rice proteomics research.
    • Understanding rice protein expression is crucial for improving this vital crop.
    • Continued application of these techniques will enhance insights into rice physiology and response to environmental factors.