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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
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Published on: December 24, 2014

Probing biofouling resistant polymer brush surfaces by atomic force microscopy based force spectroscopy.

Peter Schön1, Edit Kutnyanszky, Bas ten Donkelaar

  • 1Materials Science and Technology of Polymers, MESA(+) Institute for Nanotechnology, University of Twente, Enschede, The Netherlands.

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Summary

Zwitterionic poly(sulfobetaine methacrylate) (pSBMA) coatings effectively resist protein adsorption and barnacle larvae settlement. This antifouling property is linked to pSBMA

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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biomaterials Engineering

Background:

  • Biofouling, the accumulation of unwanted organisms on surfaces, poses significant challenges in marine environments and medical devices.
  • Zwitterionic polymers, such as poly(sulfobetaine methacrylate) (pSBMA), are known for their potential in creating antifouling surfaces.
  • Understanding protein repellency is crucial for developing effective biofouling resistance strategies.

Purpose of the Study:

  • To evaluate the protein repellency and biofouling resistance of zwitterionic pSBMA brushes.
  • To investigate the mechanism of antifouling by assessing protein adsorption and barnacle larvae settlement.
  • To correlate surface properties with antifouling performance.

Main Methods:

  • Surface-initiated polymerization (SIP) to graft pSBMA brushes onto silicon and glass substrates.
  • Atomic force microscopy (AFM) and chemical force microscopy (CFM) to measure protein adherence forces.
  • Laboratory settlement assays using cypris larvae of the barnacle Balanus amphitrite.

Main Results:

  • pSBMA surfaces significantly reduced non-specific adhesion of fibronectin, a model protein, compared to bare gold and octadecanethiol (ODT) surfaces.
  • AFM probes functionalized with arginine-glycine-aspartic acid (RGD) peptides showed no significant adhesion to pSBMA.
  • No barnacle larvae (Balanus amphitrite) adhered to pSBMA-modified surfaces in laboratory fouling studies.

Conclusions:

  • pSBMA brushes exhibit excellent protein repellency and biofouling resistance.
  • The antifouling efficacy of pSBMA is attributed to its ability to prevent non-specific protein adsorption.
  • pSBMA represents a promising material for developing advanced antifouling coatings.