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Published on: February 27, 2012
Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes
1Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK. m.d.levasseur@ncl.ac.uk
Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2012
Abstract:
Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
