Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 17, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

A Novel SNPs Detection Method Based on Gold Magnetic Nanoparticles Array and Single Base Extension.

Song Li¹, Hongna Liu, Yingying Jia

¹1. Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, P. R. China ; 2. State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering. Southeast University, Nanjing 210096, P. R. China.

|November 10, 2012

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoding adaptive immunity: advanced strategies in T and B cell repertoire analysis.

Journal of translational medicine·2026

Same author

Minimal Residual Disease Detection: Implications for Clinical Diagnosis and Cancer Patient Treatment.

MedComm·2025

Same author

Wuling capsule alleviates hyperuricaemia and protects UA- injured HK-2 cells by regulating uric acid transporter proteins.

Frontiers in pharmacology·2025

Same author

Jujube Syrup and Starter YF-L922 Co-Fermentation of Yak Yogurt: Effects of Quality Properties, Antioxidative Activities and Structure.

Food science & nutrition·2024

Same author

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing.

Frontiers in bioengineering and biotechnology·2024

Same author

Anti-oxidant effects of herbal residue from Shengxuebao mixture on heat-stressed New Zealand rabbits.

Journal of thermal biology·2024

A new high-throughput genotyping method uses gold magnetic nanoparticles (GMNPs) and single base extension for automated SNP analysis. This approach offers high sensitivity and simplifies genetic research, improving automation potential.

Area of Science:

Genetics
Biotechnology
Nanotechnology

Background:

Increasing demand for large-scale genetic research requires efficient genotyping methods.
Existing methods may lack sensitivity or require complex procedures.

Purpose of the Study:

To develop a high-throughput, automated SNP genotyping method.
To improve signal-to-noise ratio and simplify analysis for large-scale genetic studies.

Main Methods:

Utilized gold magnetic nanoparticles (GMNPs) and dual-color single base extension (SBE).
Developed a "bead array" by spotting GMNPs with fluorophore on a glass slide.
Interrogated MTHFR gene C677T polymorphism in 320 samples.

Main Results:

Keywords:

Dual-color SBE Gold magnetic nanoparticles Magnetic beads Microarray Single nucleotide polymorphisms

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Related Experiment Videos

Last Updated: May 17, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Achieved high signal/noise ratios (>12.33 for homozygous, ~1 for heterozygous).

Demonstrated high confidence in SNP loci identification.

Eliminated need for background subtraction and complex PCR product purification.

Conclusions:

The GMNP-based method provides a sensitive, automatable solution for large-scale SNP genotyping.
Simplifies the genotyping process, reducing labor and improving efficiency.
Offers a robust alternative to hybridization-based methods with superior performance.