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Updated: May 17, 2026
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Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
Published on: November 17, 2021
Evaluation of nanoparticle tracking analysis for total virus particle determination
Petra Kramberger1, Mateja Ciringer, Aleš Štrancar
1BIA Separations, d,o,o, Mirce 21, SI-5270, Ajdovščina, Slovenia. petra.kramberger@monoliths.com
Virology Journal
|November 13, 2012
Summary
Nanoparticle tracking analysis (NTA) offers a rapid method for estimating virus concentration and aggregation in various samples. This technique accurately quantifies particles like latex, adenovirus, and influenza virus, proving valuable for biological sample analysis.
Area of Science:
- Biotechnology
- Virology
- Nanotechnology
Background:
- Accurate quantification of viral particles is crucial for research and diagnostics.
- Traditional methods can be time-consuming or provide limited information on particle characteristics.
- Nanoparticle tracking analysis (NTA) presents a potential alternative for particle characterization.
Purpose of the Study:
- To evaluate the NanoSight LM10 system with Nanoparticle tracking analysis (NTA) software for quantifying biological particles.
- To assess the accuracy and inter-day variability of NTA for virus quantification.
- To compare NTA results with established methods like hemagglutination and end-point dilution assays.
Main Methods:
- Quantification of latex particles, adenovirus 5, and influenza virus using NanoSight LM10 and NTA software.
- Determination of inter-day variability by repeated measurements of the same sample.
- Validation of accuracy using known particle concentrations and comparison with hemagglutination (HA) and end-point dilution assays.
Main Results:
- NTA demonstrated accuracy in quantifying known concentrations of latex particles, adenovirus, and influenza virus.
- Inter-day variability assessments confirmed the method's reliability over consecutive days.
- NTA results correlated with traditional assays, providing insights into total and infectious virus particle numbers and aggregation levels.
Conclusions:
- Nanoparticle tracking analysis (NTA) is a viable method for the rapid estimation of virus concentration in diverse samples.
- NTA provides valuable insights into sample status, including the extent of virus aggregation.
- The NanoSight LM10 system offers a reliable tool for particle quantification in virology and biotechnology applications.
