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Updated: May 17, 2026

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
First detection of Ehrlichia canis in Rhipicephalus bursa ticks in Sardinia, Italy
Giovanna Masala1, Valentina Chisu, Cipriano Foxi
1Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy. giovanna.masala@izs-sardegna.it
Abstract:
Canine monocytic ehrlichiosis is a common and widespread disease. This disease is present in Sardinia year-round because the temperate weather on the island permits the survival of many types of tick vectors. A total of 35 ticks was collected from goats in south-eastern Sardinia, Italy, and tested for Ehrlichia DNA using polymerase chain reaction and DNA sequencing. This study provides the first evidence of the presence of Ehrlichia canis in Rhipicephalus bursa ticks. The data presented here increase our knowledge of tick-borne diseases in Sardinia and provide a useful contribution toward understanding their epidemiology. The role of R. bursa in the life cycle and transmission of E. canis needs however further investigation.

