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Updated: May 17, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Molecular characterization of a Korean bovine parainfluenza virus type 3 isolate
Jae-Ku Oem1, Eun-Yong Lee, Kyoung-Ki Lee
1Animal Disease Diagnostic Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang-6-Dong, Anyang 430-824, Republic of Korea.
Abstract:
Bovine parainfluenza virus type 3 (BPIV-3) was isolated from Korean native cattle that presented clinical signs of mild pneumonia. The complete genome of a representative isolate (12Q061) was sequenced. The newly identified strain, which was found to be distinct from the previously reported genotypes A (BPIV-3a) and B (BPIV-3b) and closely related to the Chinese strain SD0835, was tentatively classified as genotype C (BPIV-3c). Our results suggest a relationship between BPIV-3 genetic variation and the geographic location of its isolation. Identification of these new BPIV-3 genotypes may facilitate the development of improved diagnostic methods and vaccines. This is to our knowledge the first report of the identification and molecular characterization of BPIV-3 in Korea.
Insights
A new bovine parainfluenza virus type 3 (BPIV-3) genotype, BPIV-3c, was identified in Korean cattle. This discovery aids in understanding BPIV-3 genetic diversity and developing better diagnostics and vaccines.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
- Animal Health
Background:
- Bovine parainfluenza virus type 3 (BPIV-3) is a significant respiratory pathogen in cattle.
- Previous BPIV-3 classifications included genotypes A (BPIV-3a) and B (BPIV-3b).
- Limited genetic information exists for BPIV-3 strains circulating in Korea.
Purpose of the Study:
- To identify and molecularly characterize BPIV-3 strains in Korean native cattle.
- To investigate the genetic diversity and geographic distribution of BPIV-3.
- To provide foundational data for improved BPIV-3 control strategies.
Main Methods:
- Isolation of BPIV-3 from cattle with mild pneumonia symptoms.
- Whole-genome sequencing of a representative BPIV-3 isolate (12Q061).
- Phylogenetic analysis to determine the genetic relationship with known BPIV-3 genotypes.
Main Results:
- A novel BPIV-3 strain, designated 12Q061, was isolated from Korean native cattle.
- The identified strain was genetically distinct from BPIV-3a and BPIV-3b.
- The new strain showed close relation to a Chinese isolate and was tentatively classified as genotype C (BPIV-3c).
- Results suggest a correlation between BPIV-3 genetic variation and geographical origin.
Conclusions:
- This study reports the first identification and molecular characterization of BPIV-3 in Korea.
- The discovery of BPIV-3c expands the known genetic diversity of the virus.
- Understanding BPIV-3 genetic variation is crucial for developing effective diagnostic tools and vaccines.
