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Updated: May 17, 2026

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

Bisphenol A-glucuronide measurement in urine samples.

Catherine Harthé¹, Sabina Rinaldi, David Achaintre

¹Laboratoire d'Hormonologie, Centre de Biologie et de Pathologie Est, Groupement Hospitalier Est, Hospices Civils de Lyon, France. catherine.harthe@chu-lyon.fr

|November 13, 2012

Summary

This study developed a radioimmunoassay to measure Bisphenol A-glucuronide (BPA-G), the main BPA metabolite in urine. The method accurately detects BPA-G, addressing contamination issues and confirming widespread BPA exposure in the human population.

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Area of Science:

Environmental Health
Analytical Chemistry
Endocrinology

Background:

Bisphenol A (BPA) is a prevalent environmental endocrine disruptor found in human samples.
Concerns exist regarding BPA measurement accuracy due to sample collection and laboratory contamination.
BPA-glucuronide (BPA-G) is the primary endogenous metabolite of BPA in urine.

Purpose of the Study:

To develop and validate a specific radioimmunoassay (RIA) for quantifying BPA-G in human urine.
To overcome challenges associated with measuring unconjugated BPA and potential laboratory contamination.
To assess human exposure to BPA by measuring its main metabolite.

Main Methods:

A polyclonal anti-BPA antibody with high cross-reactivity to BPA-G was utilized.
An extraction step using dichloromethane was implemented to remove unconjugated BPA.
The RIA method was validated for precision and accuracy within the 0.05–5 μg/L range.

Main Results:

The developed RIA method demonstrated validity, precision, and accuracy.
BPA-G was detected in all 163 urine samples analyzed from a hospital population.
The mean concentration of BPA-G detected was 4.64 μg/L.

Conclusions:

The developed radioimmunoassay is a valuable tool for screening BPA exposure in human populations.
This method effectively addresses potential contamination issues during laboratory analysis.
The findings confirm the ubiquitous presence of BPA exposure, as indicated by its primary metabolite in urine.