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A High-throughput Shigella-specific Bactericidal Assay
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Functional assay for shiga-like toxin via detection by antibody capture and multivalent galabiose binding

Hilde van Hattum1, Kim van der Zwaluw, Gerben M Visser

  • 1Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands.

Bioorganic & Medicinal Chemistry Letters
|November 13, 2012
PubMed

Keywords:
bacterial diagnosticsprotein activity assayclinical microbiology toolspathogen detection methods

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.