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Related Experiment Video

Updated: May 17, 2026

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

Charting monosynaptic connectivity maps by two-color light-sheet fluorescence microscopy.

Christian J Niedworok¹, Inna Schwarz, Julia Ledderose

¹Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany.

|November 13, 2012

Summary

Researchers developed a new viral method to map neuronal connections in the adult mouse brain. This technique visualizes long-range neuronal networks and their direct connections at cellular resolution, aiding in understanding neural information flow.

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Last Updated: May 17, 2026

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

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Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

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Published on: February 22, 2015

Area of Science:

Neuroscience
Molecular Biology
Imaging Techniques

Background:

Mapping neuronal circuitry is crucial for understanding brain function.
Visualizing long-range neuronal networks in intact adult brains at cellular resolution remains challenging.

Purpose of the Study:

To develop a novel viral strategy for fluorescently labeling neuronal populations and their monosynaptic connections.
To enable high-resolution 3D visualization of neural networks in the uncut adult mouse brain.

Main Methods:

A virus-based strategy was employed for stereotaxic injection into specific neuronal populations.
Improved tissue clearing and light-sheet fluorescence microscopy were used for whole-brain imaging.
Rehydration of cleared brains allowed for subsequent immunohistochemical identification of connected neurons.

Main Results:

Successful fluorescent labeling of centrifugally projecting neurons and their monosynaptically connected bulbar interneurons.
Detailed 3D reconstructions revealed the distribution of neuronal ensembles connected to specific bulbar interneuron populations.
Demonstrated feasibility of post-imaging immunohistochemistry for synaptic identification.

Conclusions:

The described method provides a powerful tool for mapping monosynaptic connectivity.
This approach facilitates a deeper understanding of information flow within neural systems.
Enables visualization of neural networks in the intact adult mouse brain at cellular resolution.