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Updated: May 17, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
Systematic reconstruction of RNA functional motifs with high-throughput microfluidics
Lance Martin1, Matthias Meier, Shawn M Lyons
1Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
Abstract:
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.

