Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute and Chronic Myocarditis in Men and Women.

Current heart failure reports·2026
Same author

Rewiring Intercellular Communication with Self-Assembling Nanofibers.

ACS nano·2026
Same author

High-speed hyperspectral single-pixel microscopy via line-scan detection with data fusion-based enhanced resolution.

Communications engineering·2026
Same author

Correction: Electrospun polymeric scaffolds enable 3D tissue-like functionality and efficient photoinduced contraction.

Journal of materials chemistry. B·2026
Same author

Computational 3D multispectral fluorescence lifetime microscopy.

Optics express·2026
Same author

Phantom for standardization in functional near-infrared spectroscopy, part 2: optical properties and Monte Carlo simulations.

Neurophotonics·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: May 17, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Absorption spectroscopy of powdered materials using time-resolved diffuse optical methods.

Cosimo D'Andrea1, Ekaterina A Obraztsova, Andrea Farina

  • 1Dipartimento di Fisica, Politecnico di Milano, Milan, Italy. cosimo.dandrea@polimi.it

Applied Optics
|November 13, 2012
PubMed
Summary
This summary is machine-generated.

A new time-resolved diffuse optical spectroscopy method accurately measures nanostructured powder absorption, even with scattering. This technique was validated on India Ink particles and carbon nanotubes, showing effective scattering-free absorption measurements.

More Related Videos

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
10:13

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

Published on: April 28, 2023

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Related Experiment Videos

Last Updated: May 17, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
10:13

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks

Published on: April 28, 2023

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Area of Science:

  • Materials Science
  • Optical Spectroscopy
  • Nanotechnology

Background:

  • Accurate absorption measurement of nanostructured powders is challenging due to significant light scattering.
  • Conventional spectrophotometry methods can be confounded by scattering effects in powdered samples.
  • Developing scattering-independent techniques is crucial for material characterization.

Purpose of the Study:

  • To propose and validate a novel method for measuring the absorption of nanostructured powders.
  • To achieve scattering-free absorption measurements in the visible and near-infrared spectral ranges.
  • To compare the proposed technique with conventional spectrophotometry.

Main Methods:

  • Utilizing time-resolved diffuse optical spectroscopy (TR-DOS).
  • Developing a method to isolate absorption signals from scattering effects.
  • Conducting experimental validation using India Ink particles and carbon nanotubes.

Main Results:

  • The proposed TR-DOS method successfully measured scattering-free absorption spectra of nanostructured materials.
  • Validation experiments on India Ink particles demonstrated the technique's efficacy.
  • Carbon nanotubes were used to further confirm the method's ability to provide accurate absorption data independent of scattering.

Conclusions:

  • Time-resolved diffuse optical spectroscopy offers a robust solution for measuring the absorption of nanostructured powders.
  • The developed method overcomes limitations of conventional techniques by eliminating scattering interference.
  • This advancement facilitates more accurate material characterization and analysis in nanotechnology and materials science.