Related Experiment Video
Updated: May 17, 2026
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
Published on: June 13, 2025
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila
Satoshi Fukuhara1, Hiroshi Nishimasu, Luc Bonnefond
1Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan.
Abstract:
PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive. Drosophila melanogaster Zuc (DmZuc) was expressed in Escherichia coli, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a=55.0, b=71.2, c=56.3 Å, β=107.9°.
