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Real-time polymerase chain reaction for diagnosing acyclovir-resistant herpetic keratitis based on changes in viral

Tomoyuki Inoue1, Rumi Kawashima, Takashi Suzuki

  • 1Department of Ophthalmology, Ehime University School of Medicine and Department of Ophthalmology, Sumitomo Hospital, Osaka, Japan. tomonoue@gmail.com

Archives of Ophthalmology (Chicago, Ill. : 1960)
|November 13, 2012
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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