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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview

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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

A simple and rapid method for determining transgenic cotton plants.

Baohong Zhang1, Hongmei Wang, Fang Liu

  • 1Department of Biology, East Carolina University, Greenville, NC, USA. zhangb@ecu.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2012
PubMed
Summary

A new, nondestructive bioassay rapidly identifies transgenic cotton plants using chemical sensitivity. This method is cost-effective and speeds up the application of transgenic technology in cotton breeding.

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Area of Science:

  • Agricultural biotechnology
  • Plant genetics

Background:

  • Accurate determination of transgenic events is crucial for developing and applying genetically modified crops.
  • Conventional methods like Northern blotting and qRT-PCR are time-consuming, expensive, and destructive.

Purpose of the Study:

  • To present a simple, rapid, and nondestructive method for identifying transgenic cotton plants.
  • To reduce the cost and time associated with screening transgenic events.

Main Methods:

  • Utilizing the differential sensitivity of transgenic and non-transgenic cotton plants to specific chemicals (e.g., antibiotics, herbicides).
  • Implementing a bioassay suitable for both laboratory and field conditions.

Main Results:

  • The developed method allows for quick and cost-effective screening of transgenic cotton.
  • The bioassay is nondestructive, enabling subsequent cultivation of confirmed transgenic plants.

Conclusions:

  • This nondestructive bioassay significantly facilitates the screening of transgenic cotton events.
  • The method accelerates the adoption of transgenic technology in cotton breeding and production by saving resources.