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Related Concept Videos

Gene Flow02:39

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview

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Related Experiment Video

Updated: May 17, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

Determining gene flow in transgenic cotton.

Xiaoping Pan1

  • 1Department of Biology, East Carolina University, Greenville, NC, USA. panx@ecu.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2012
PubMed
Summary
This summary is machine-generated.

Evaluating gene flow from transgenic cotton is crucial to prevent environmental risks like superweeds. This study presents a practical field method to assess transgene spread in insect-resistant and herbicide-tolerant cotton varieties.

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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Last Updated: May 17, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Area of Science:

  • Agricultural Science
  • Environmental Science
  • Genetics

Background:

  • Gene flow from transgenic plants poses environmental risks, including the development of herbicide-resistant weeds and reduced genetic diversity.
  • Transgenic crops, such as insect-resistant and herbicide-tolerant cotton, are widely cultivated globally.
  • Assessing the extent of gene flow is essential for risk management and regulatory approval of genetically modified organisms (GMOs).

Purpose of the Study:

  • To present a practical field methodology for determining transgene flow in cotton.
  • To provide a framework for evaluating gene flow from commercially relevant transgenic cotton varieties.
  • To address concerns regarding the environmental impact of genetically modified crops.

Main Methods:

  • The study outlines a three-part field practice method for assessing transgene flow.
  • Key components include meticulous field design, systematic seed collection strategies, and robust field and laboratory bioassays.
  • Specific examples utilize transgenic insect-resistant and herbicide-tolerant cotton.

Main Results:

  • The described methodology enables the quantification and characterization of transgene movement in agricultural settings.
  • This approach facilitates the evaluation of gene flow extent under real-world field conditions.
  • The study provides a reproducible protocol for monitoring transgenic crop gene flow.

Conclusions:

  • The presented field practice method is effective for determining transgene flow in cotton.
  • This methodology is vital for environmental risk assessment of transgenic crops.
  • Implementing such field evaluations is necessary for the responsible deployment of genetically modified agricultural technologies.