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In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

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Embedding resorcinarene cavitands in lipid vesicles().

Katie M Feher¹, Hai Hoang, Michael P Schramm

¹California State University Long Beach, Department of Chemistry and Biochemistry, 1250 Bellflower Blvd., Long Beach, CA 90840, USA; ; Tel: +1 562-985-1866.

New Journal of Chemistry = Nouveau Journal De Chimie

|November 13, 2012

Summary

A fluorescently labeled resorcinarene cavitand was embedded within DLPC lipid vesicles. Confocal microscopy confirmed the cavitand exclusively resides within the lipid bilayer.

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Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Area of Science:

Supramolecular Chemistry
Biophysical Chemistry
Materials Science

Background:

Cavitands are macrocyclic molecules with unique host-guest properties.
Lipid vesicles are common models for biological membranes.
Understanding molecular interactions within lipid bilayers is crucial for drug delivery and biomaterials.

Purpose of the Study:

To investigate the incorporation and localization of a fluorescently labeled resorcinarene cavitand within lipid vesicles.
To visualize the cavitand's spatial distribution within the lipid bilayer using advanced imaging techniques.

Main Methods:

Synthesis of a fluorescently labeled resorcinarene cavitand.
Preparation of 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) lipid vesicles.
Confocal fluorescence microscopy for imaging and localization studies.

Main Results:

Successful embedding of the fluorescent cavitand into DLPC lipid vesicles.
Confocal microscopy demonstrated exclusive localization of the cavitand within the lipid bilayer.
No significant aggregation or disruption of the vesicle structure was observed.

Conclusions:

Resorcinarene cavitands can be effectively incorporated into lipid bilayers.
The cavitand maintains its structural integrity and resides exclusively within the membrane.
This finding supports the potential use of cavitands in membrane-associated applications.