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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Computed Tomography

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Two-Dimensional Microscopy in Microbiology

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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Molecular imaging true-colour spectroscopic optical coherence tomography.

Francisco E Robles1, Christy Wilson, Gerald Grant

  • 1Department of Biomedical Engineering and Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA ; Medical Physics Program, Duke University, Durham, North Carolina 27708, USA.

Nature Photonics
|November 13, 2012
PubMed
Summary

A new spectroscopic optical coherence tomography method enables molecular imaging of endogenous and exogenous chromophores. This technique provides high-resolution, true-colour 3D images, aiding in disease diagnosis and understanding.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Spectroscopy

Background:

  • Molecular imaging is crucial for understanding disease mechanisms at cellular and molecular levels.
  • Existing techniques like fluorescence imaging have limitations in penetration depth and agent specificity.
  • Optical coherence tomography (OCT) offers high resolution but often lacks detailed molecular information.

Purpose of the Study:

  • To develop a novel spectroscopic optical coherence tomography technique for enhanced molecular imaging.
  • To demonstrate the capability of imaging both endogenous and exogenous chromophores with high fidelity.
  • To enable true-colour representation and assessment of physiological parameters like hemoglobin oxygenation.

Main Methods:

  • Utilized a wide spectral bandwidth laser source centered in the visible spectrum.
  • Developed a spectroscopic optical coherence tomography approach named METRiCS OCT.
  • Achieved micrometre-scale resolution in three dimensions with high spectral fidelity.

Main Results:

  • Successfully demonstrated molecular imaging of endogenous and exogenous chromophores.
  • Enabled facile assessment of hemoglobin oxygen levels, providing contrast from absorbers.
  • Achieved true-colour representation of samples with high spectral fidelity.

Conclusions:

  • METRiCS OCT offers a powerful new tool for molecular imaging in biomedical applications.
  • The technique has significant implications for ophthalmology, early cancer detection, and studying hypoxia and angiogenesis.
  • This approach enhances diagnostic capabilities by providing detailed molecular and physiological insights.