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Updated: May 17, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

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Published on: December 29, 2021

iNuc-PhysChem: a sequence-based predictor for identifying nucleosomes via physicochemical properties.

Wei Chen¹, Hao Lin, Peng-Mian Feng

¹Department of Physics, School of Sciences, Center for Genomics and Computational Biology, Hebei United University, Tangshan, China. wchen@gordonlifescience.org

|November 13, 2012

Summary

This study identifies key DNA physicochemical features that define nucleosome positioning in yeast. A new predictor, iNuc-PhysChem, accurately distinguishes nucleosomal sequences, aiding research.

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Published on: September 21, 2017

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Nucleosome positioning is crucial for cellular processes.
Predicting nucleosome positioning remains a challenge due to elusive rules.

Purpose of the Study:

To systematically compare DNA physicochemical features in nucleosomal and linker sequences.
To develop a computational tool for identifying nucleosomal sequences.

Main Methods:

Comparative analysis of twelve DNA physicochemical features in Saccharomyces cerevisiae.
Development of the iNuc-PhysChem predictor using feature vectors.
Optimization of feature sets using incremental feature selection (IFS).

Main Results:

Identified position-specific physicochemical features within nucleosomal sequences.
iNuc-PhysChem achieved over 96% accuracy in distinguishing nucleosomal from linker DNA.
A user-friendly web server and downloadable code were made available.

Conclusions:

Physicochemical properties can effectively predict nucleosome occupancy.
iNuc-PhysChem offers a high-throughput tool for biological research and drug design.
The predictor enhances the study of nucleosome organization and function.