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A Murine Model of Group B Streptococcus Vaginal Colonization
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Published on: November 16, 2016

Structural basis for group B streptococcus pilus 1 sortases C regulation and specificity.

Roberta Cozzi1, Daniil Prigozhin, Roberto Rosini

  • 1Novartis Vaccines and Diagnostics, Siena, Italy.

Plos One
|November 13, 2012
PubMed
Summary

Class C sortase enzymes build pili in Gram-positive bacteria. Researchers determined the crystal structures of two Group B Streptococcus sortases, revealing a conserved catalytic core and a flexible lid region potentially influencing substrate specificity.

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Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Gram-positive bacteria utilize class C sortase enzymes for pilus assembly, polymerizing pilin subunits into elongated structures.
  • Pilus assembly is crucial for bacterial adhesion and virulence.

Purpose of the Study:

  • To elucidate the structural basis of class C sortase function in pilus assembly.
  • To investigate the role of the N-terminal lid region in sortase activity and specificity.

Main Methods:

  • Crystal structure determination of SrtC1 and SrtC2 from Group B Streptococcus.
  • In vitro enzymatic assays using recombinant sortase variants.
  • Förster resonance energy transfer (FRET)-based assays to study protein interactions.

Main Results:

  • The crystal structures revealed a conserved 8-stranded β-barrel catalytic core and a flexible N-terminal lid domain for both SrtC1 and SrtC2.
  • The N-terminal lid region was found to be dispensable for catalytic activity but may play a role in substrate recognition and regulation.
  • FRET assays indicated that the LPXTG motif is not the sole determinant for sortase C specificity in pilin recognition.

Conclusions:

  • The structural and functional data provide insights into the mechanism of pilus assembly by class C sortases.
  • The N-terminal lid region represents a potential target for modulating sortase activity and specificity.
  • Understanding sortase specificity is crucial for developing targeted antibacterial strategies.