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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Published on: July 10, 2016

Passive circulating cell sorting by deformability using a microfluidic gradual filter.

P Preira1, V Grandné, J-M Forel

  • 1Adhésion & Inflammation, INSERM U1067-CNRS UMR7333, and Université Aix-Marseille, Assistance Publique-Hôpitaux de Marseille, Case 937, 163 Avenue de Luminy, F-13009 Marseille, France.

Lab on a Chip
|November 14, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic device to measure cell deformability, crucial for diseases like sepsis and acute respiratory distress syndrome (ARDS). The method passively sorts cells based on their stiffness, offering a new diagnostic tool.

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A Microfluidic Technique to Probe Cell Deformability
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A Microfluidic Technique to Probe Cell Deformability
09:47

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Pathophysiology

Background:

  • Cell deformability is a key factor in diseases such as sepsis and acute respiratory distress syndrome (ARDS).
  • Assessing cell deformability is vital for understanding disease mechanisms and developing treatments.

Purpose of the Study:

  • To present a novel microfluidic method for passive testing, sorting, and separating non-adherent cells based on their deformability.
  • To demonstrate the method's capability in analyzing cell stiffness using various treatments and patient samples.

Main Methods:

  • A microfluidic device utilizing a series of sieves with progressively decreasing pore sizes.
  • Passive cell separation based on deformability without active cell manipulation.
  • Application of monocytic cell lines (THP-1) treated with actin-modulating drugs (Jasplakinolide, LatrunculinA) and plasma from ARDS patients.

Main Results:

  • The microfluidic method successfully sorts and separates cells based on their deformability.
  • Demonstrated efficacy with treated cell lines and clinical samples from ARDS patients.
  • The system allows for passive analysis of cell mechanical properties.

Conclusions:

  • The developed microfluidic sieving technique offers a viable method for assessing cell deformability.
  • This approach has potential applications in diagnosing and understanding diseases like ARDS and sepsis.
  • The method's adaptability for hospital settings due to simple sample injection is highlighted.