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Functional insights into the core-TFIIH from a comparative survey
Florence Bedez1, Benjamin Linard, Xavier Brochet
1Laboratoire de Bioinformatique et Génomique Intégratives, Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS, INSERM, UDS), BP163, 67404 Illkirch Cedex, France.
Genomics
|November 14, 2012
Summary
The core transcription factor II H (TFIIH) complex, vital for DNA repair and transcription, shows varied presence of some subunits in unicellular organisms. This suggests potential roles in mRNA splicing for subunit P34.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- TFIIH is a eukaryotic transcription factor complex.
- It consists of the CAK and core-TFIIH subcomplexes.
- Core-TFIIH has seven subunits and is essential for transcription and DNA repair.
Purpose of the Study:
- To determine the phylogenetic distribution of core-TFIIH subunits across 63 eukaryotic species.
- To investigate the roles of core-TFIIH subunits through comparative proteome analysis.
- To identify potential new functions of core-TFIIH subunits.
Main Methods:
- Extended sequence analysis of core-TFIIH subunits.
- Phylogenetic distribution analysis in 63 eukaryotic organisms.
- Comparative genomic analysis of whole proteomes.
Main Results:
- Seven core-TFIIH subunits are highly conserved across eukaryotes.
- Non-enzymatic subunits P8, P34, P52, and P62 are absent in some unicellular species.
- P52's role in repair is confirmed; P34 is implicated in mRNA splicing.
Conclusions:
- Core-TFIIH subunit presence varies in unicellular eukaryotes.
- Comparative genomics reveals functional insights into TFIIH subunits.
- P34 subunit of core-TFIIH may play a role in mRNA splicing.
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