Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raf-like protein kinase heterocomplexes directly regulate the plant plasma membrane H<sup>+</sup>-ATPase.

Science (New York, N.Y.)Ā·2026
Same author

Live-cell tracking of biliverdin trafficking reveals metabolic exchange between plastids and peroxisomes.

Biology openĀ·2026
Same author

Wounding activates the HSFA1 transcription factors to promote cellular reprogramming in Arabidopsis.

The Plant cellĀ·2026
Same author

Phototropin can trigger the chloroplast accumulation response from multiple subcellular locations in Marchantia polymorpha.

Biochemical and biophysical research communicationsĀ·2026
Same author

Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening.

Nature communicationsĀ·2026
Same author

Strawberry atlas: <i>Fragaria vesca</i> gene expression atlas for strawberry genomics.

PeerJĀ·2026

Related Experiment Video

Updated: May 16, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives.

Yutaka Kodama1, Chang-Deng Hu

  • 1Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan. kodama@cc.utsunomiya-u.ac.jp

Biotechniques
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Bimolecular fluorescence complementation (BiFC) visualizes protein interactions. Recent improvements enhance BiFC technology for better signal and new approaches, aiding protein interaction studies.

More Related Videos

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
11:10

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Published on: March 9, 2014

Related Experiment Videos

Last Updated: May 16, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
11:10

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Published on: March 9, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Bimolecular fluorescence complementation (BiFC) is a key technique for visualizing protein-protein interactions in model organisms.
  • The assay relies on the reconstitution of a fluorescent protein from non-fluorescent fragments upon protein interaction.

Purpose of the Study:

  • To review current advancements in BiFC technology.
  • To discuss improvements in BiFC systems, fluorescent protein split sites, and signal-to-noise ratios.
  • To provide perspectives on future developments in BiFC techniques.

Main Methods:

  • Review of existing literature on BiFC technology.
  • Analysis of BiFC system development and improvements.
  • Examination of fluorescent protein split site research.
  • Evaluation of signal-to-noise ratio enhancements.

Main Results:

  • BiFC technology has undergone significant improvements over the past decade.
  • New approaches and enhanced BiFC systems are emerging.
  • Understanding of split sites and signal-to-noise ratio optimization are key areas of development.

Conclusions:

  • BiFC is a valuable tool for studying protein interactions.
  • Ongoing technological advancements promise more sensitive and versatile BiFC assays.
  • Future research will likely focus on further refining BiFC for diverse physiological conditions.