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Epitope mapping

S E Mole1

  • 1CRC Human Cancer Genetics Research Group, Department of Pathology, University of Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...