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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

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Immunochemical applications of avidin-biotin technology

E A Bayer1, M Wilchek

  • 1Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary

Avidin-biotin technology enhances antigen purification by minimizing antibody damage during immobilization. This method uses avidin as a stable buffer, protecting antibodies from detrimental interactions with solid supports.

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