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Updated: May 16, 2026

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Published on: April 17, 2018
The intracellular metabolism of legionella by isotopologue profiling
Klaus Heuner1, Wolfgang Eisenreich
1Working group "Cellular Interactions of Bacterial Pathogens", Robert Koch-Institut, Berlin, Germany. heunerk@rki.de
Abstract:
Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general (13)C-labeled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.
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