Related Experiment Video
Updated: May 16, 2026
06:51
Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
Autophagy, senescence, and apoptosis
Rachel W Goehe1, Molly L Bristol, Eden N Wilson
1Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
Summary
This chapter details methods to study the p53 protein
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The p53 protein is a critical tumor suppressor involved in cellular stress responses.
- p53 plays a central role in regulating cell fate following DNA damage, influencing apoptosis, autophagy, and senescence.
- Understanding p53's function is crucial for developing targeted cancer therapies.
Purpose of the Study:
- To present updated methodologies for investigating p53's role in apoptosis, autophagy, and senescence.
- To provide researchers with practical protocols for studying p53 signaling pathways.
- To enable quantitative and efficient analysis of p53's involvement in cellular stress responses.
Main Methods:
- Utilizing quantitative and efficient in vitro assays.
- Applying up-to-date procedures for molecular network analysis.
- Interrogating p53 signaling pathways through various experimental approaches.
Main Results:
- Established methods allow for precise examination of p53's influence on cell fate decisions.
- The presented techniques overcome limitations of previous methodologies.
- Researchers can now efficiently study p53's role in adaptive stress responses and programmed cell death.
Conclusions:
- The chapter provides essential tools for dissecting p53's complex regulatory functions.
- These methods facilitate a deeper understanding of how p53 governs cell survival or death under stress.
- This work supports advancements in cancer research and therapeutic strategies targeting the p53 pathway.
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