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Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

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Related Experiment Video

Updated: May 16, 2026

Cell Cycle-specific Measurement of &#947;H2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

p53 and cell cycle effects after DNA damage.

Emir Senturk1, James J Manfredi

  • 1Mount Sinai School of Medicine, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Flow cytometry analyzes cell cycle DNA content to understand DNA damage response. This method reveals how p53 influences cell cycle checkpoints, impacting DNA repair and mutation prevention.

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Last Updated: May 16, 2026

Cell Cycle-specific Measurement of &#947;H2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Cell cycle checkpoints are crucial for preventing mutations during DNA repair.
  • Flow cytometry is a powerful technique for analyzing individual cell properties.

Purpose of the Study:

  • To evaluate the role of p53 in cell cycle checkpoints after DNA damage.
  • To demonstrate the utility of flow cytometry in assessing DNA content and cell cycle phase distribution.

Main Methods:

  • Cells were permeabilized and stained with fluorescent dyes to measure DNA content.
  • Flow cytometry was used to analyze light scattering and fluorescence emission.
  • Cell cycle phases (G1, S, G2/M) were quantified based on DNA content.

Main Results:

  • Distinguished cells with 2N DNA content (G1) from those with 4N DNA content (G2).
  • Observed changes in cell cycle profiles (G1, S, G2/M phases) following DNA damage.
  • Demonstrated the ability to assess DNA damage-induced cell cycle arrest patterns.

Conclusions:

  • Flow cytometry is effective for analyzing cell cycle checkpoints in response to DNA damage.
  • The technique allows for the evaluation of p53's role in DNA damage-induced cell cycle arrest.