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Measurement of chemosensitivity and growth rate in p53 expressing cells
Mahesh Ramamoorthy1, Catherine Vaughan, Sumitra Deb
1Laboratory of Molecular Gerontology, National Institute on Aging, Biomedical Research Center, Baltimore, MD, USA.
Abstract:
Chemoresistance and increased growth rate are two gain-of-function functions that mutant p53 is thought to possess. Here, we describe two methods for measuring the sensitiveness of cells to chemotherapeutic drugs and the rate of cell growth. Both of which can be used with a wide range of cell types. The clonogenic assay can be used with many different chemotoxic drugs and the growth assay described here presents an alternative to the MTT assay and allows for a long-term measurement of cell growth. These protocols are both easy, flexible, require relatively little effort, and are inexpensive to carry out.
