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Updated: May 16, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Challenges and opportunities for small molecule aptamer development.

Maureen McKeague1, Maria C Derosa

  • 1Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6.

Journal of Nucleic Acids
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

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Developing aptamers for small molecules presents challenges but offers opportunities for biosensing and chemical biology. These DNA or RNA probes show high affinity for targets, aiding diagnostics and therapeutics.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Aptamers are single-stranded DNA or RNA molecules that bind targets with high affinity and selectivity.
  • They are increasingly used in biosensing, diagnostics, and therapeutics.
  • However, developing aptamers for small molecule targets remains a significant challenge.

Purpose of the Study:

  • To highlight the technical challenges in developing aptamers for small molecule targets.
  • To explore the opportunities for aptamer applications in biosensing and chemical biology for small molecules.

Main Methods:

  • The paper reviews existing literature and technical considerations for aptamer selection and development.
  • It focuses on strategies to overcome challenges specific to small molecule aptamerization.

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Related Experiment Videos

Last Updated: May 16, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Main Results:

  • Despite difficulties, aptamer development for small molecules is feasible.
  • Successful aptamers can serve as novel molecular recognition probes.

Conclusions:

  • Aptamers offer a promising avenue for detecting and interacting with small molecules.
  • Further research into aptamer development for small molecules will advance biosensing and chemical biology applications.