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Updated: May 16, 2026

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
Published on: March 22, 2019
Structural studies of human insulin cocrystallized with phenol or resorcinol via powder diffraction
Fotini Karavassili1, Anastasia E Giannopoulou, Eleni Kotsiliti
1Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
Abstract:
The effects of the ligands phenol and resorcinol on the crystallization of human insulin have been investigated as a function of pH. Powder diffraction data were used to characterize several distinct polymorphic forms. A previously unknown polymorph with monoclinic symmetry (P2(1)) was identified for both types of ligand with similar characteristics [the unit-cell parameters for the insulin-resorcinol complex were a = 114.0228 (8), b = 335.43 (3), c = 49.211 (6) Å, β = 101.531 (8)°].
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