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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

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ADAMTS: novel proteases expressed by activated mast cells

Gianni García-Faroldi1, Elin Rönnberg, Adolfo Orro

  • 1Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, Uppsala, Sweden. Gianni.Garcia@slu.se

Biological Chemistry
|November 17, 2012
PubMed

Insights

Mast cells (MCs) express metalloproteases from the ADAMTS family. MC activation, influenced by bacteria or immune signals, alters ADAMTS expression, suggesting their role in MC function.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Mast cells (MCs) are key immune cells involved in allergic responses and inflammation.
  • The A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family comprises proteases with diverse biological functions.
  • The role of ADAMTS proteases in mast cell biology remains largely unexplored.

Purpose of the Study:

  • To investigate the expression of ADAMTS proteases in mast cells.
  • To determine how mast cell activation influences ADAMTS expression.
  • To explore the functional implications of ADAMTS proteases in mast cells.

Main Methods:

  • Co-culture of mast cells with Gram-positive bacteria.
  • Mast cell activation using calcium ionophore and IgE receptor crosslinking.
  • Analysis of ADAMTS gene and protein expression via quantitative PCR and immunocytochemistry.
  • Investigation of signaling pathways involved in ADAMTS regulation.

Main Results:

  • Mast cells express multiple ADAMTS proteases, including ADAMTS-5, -6, and -9.
  • Mast cell activation profoundly impacts ADAMTS expression, inducing ADAMTS-9 and -6 while down-regulating ADAMTS-5.
  • ADAMTS expression is closely linked to mast cell maturation and exhibits aggrecanase activity.

Conclusions:

  • Mast cells express and regulate the expression of ADAMTS proteases.
  • Mast cell activation influences the profile of ADAMTS proteases, implicating them in mast cell function.
  • These findings highlight a novel role for ADAMTS proteases in mast cell biology and immune responses.

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