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Updated: May 16, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Discrimination of mushrooms causing food-poisoning incidents by using DNA sequence analysis
Atsushi Masayama1, Taro Murakami, Daisuke Sakuma
1Osaka City Institute of Public Health and Environmental Science, Osaka, Japan.
Abstract:
In this study, the identification of mushrooms by using DNA analysis was investigated. Our analysis of internal transcribed spacer (ITS) regions revealed that a DNA-based method could be applicable for samples that are difficult to distinguish in terms of the morphological characteristics. PCR amplification using templates extracted from cooked samples gave sufficient fragments to analyze the sequence. However, treatment with simulated gastric fluid (SGF) for more than 30 min affected the analysis of the ITS region. Application to samples of vomit is also discussed.
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