Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stratum corneum lipid remodeling as a proposed mechanism for liposomal penetration enhancement.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026

Same author

Modular Expression of <i>Botryococcus braunii</i> Genes Enhances Isoprenoid Production in the Diatom <i>Phaeodactylum tricornutum</i>.

ACS synthetic biology·2026

Same author

PAF15-PCNA exhaustion governs the strand-specific control of DNA replication.

Nature·2026

Same author

Real-Time Tracking of Intracellular Prenyl Phosphate Pools in the Marine Diatom <i>Phaeodactylum tricornutum</i> with a Metabolite Protein-Based Biosensor.

ACS synthetic biology·2026

Same author

Hepatic stellate cells regulate liver fatty acid utilization via plasmalemma vesicle-associated protein.

Cell metabolism·2025

Same author

Stochastic demethylation and redundant epigenetic suppressive mechanisms generate highly heterogeneous responses to pharmacological DNA methyltransferase inhibition.

Journal of experimental & clinical cancer research : CR·2025

Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026

Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026

Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026

Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026

Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026

Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 1, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Multi-color single particle tracking with quantum dots.

Eva C Arnspang¹, Jonathan R Brewer, B Christoffer Lagerholm

¹Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark.

|November 17, 2012

Summary

This summary is machine-generated.

Quantum dots (QDs) offer advanced fluorescence detection for multiplex single molecule applications. This study quantifies QD properties, enabling multicolor single particle tracking in live cells.

More Related Videos

Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Related Experiment Videos

Last Updated: May 1, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Area of Science:

Nanotechnology
Biophysics
Spectroscopy

Background:

Quantum dots (QDs) offer unique optical properties for sensitive fluorescence detection.
Multiplex single molecule applications, like single particle tracking (SPT), require optimized QD performance.
Current QD applications have not fully exploited their potential for simultaneous multi-species detection.

Purpose of the Study:

To comprehensively investigate fluorescence intensities, fluctuations, and hydrodynamic radii of commercially available water-soluble QDs.
To optimize QDs for multiplex single molecule sensitivity applications.
To demonstrate multicolor single particle tracking (MC-SPT) with QDs.

Main Methods:

Quantitative investigation of eight types of commercially available water-soluble QDs.
Analysis of fluorescence intensity, fluorescence intensity fluctuations, and hydrodynamic radii.
Demonstration of four-color MC-SPT at an image acquisition rate of 25 Hz.

Main Results:

CdSe core QDs show increased fluorescence intensity with red-shifted emission; CdSe/CdTe core QDs are less bright than red-shifted CdSe QDs.
Blue-shifted QDs offer minimal size advantage in biological applications due to surface coatings.
Parallel four-color MC-SPT is feasible with QDs at >= 25 Hz acquisition rate.

Conclusions:

Optimized QDs enable enhanced multiplex single molecule detection.
Understanding QD optical and physical properties is crucial for advanced applications.
MC-SPT with QDs is a viable technique for studying cellular dynamics in live cells.