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Dysplasia-carcinoma transition specific transcripts in colonic biopsy samples
Orsolya Galamb1, Barnabás Wichmann, Ferenc Sipos
12nd Department of Medicine, Semmelweis University, Budapest, Hungary.
Plos One
|November 17, 2012
Summary
Researchers identified 11 key transcripts that accurately detect the transition from dysplasia to colorectal cancer (CRC) in biopsies. This molecular marker set offers objective classification for benign and malignant colorectal diseases.
Area of Science:
- Molecular biology
- Genomics
- Oncology
Background:
- Early molecular detection of dysplasia-carcinoma transition can improve colonic biopsy diagnosis.
- Objective classification of benign and malignant colorectal diseases is needed.
Purpose of the Study:
- Identify characteristic transcript sets for diagnostic mRNA expression patterns.
- Develop objective classification for benign and malignant colorectal diseases.
- Test the classificatory power of these markers on independent samples.
Main Methods:
- Utilized HGU133plus2 microarrays to identify CRC and adenoma-specific transcript sets.
- Analyzed 94 independent biopsies using microarrays to test discriminatory genes.
- Validated findings with array real-time PCR on 68 independent samples.
Main Results:
- Identified a set of 11 transcripts (including CXCL1, CHI3L1, GREM1) that discriminate high-grade dysplastic adenoma from CRC with 100% sensitivity and 88.9% specificity.
- Demonstrated high discriminatory power on independent samples via microarray and RT-PCR.
- Achieved 95.6% correct classification in original samples and 94.1% in cross-validated samples.
Conclusions:
- The identified transcripts accurately characterize the dysplasia-carcinoma transition in biopsy samples.
- These markers provide a basis for gene expression-based diagnostic classification of colorectal cancer.
- Diagnostic RT-PCR cards could be integrated into automated routine procedures.
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