[Physiological effects induced by dermorphin synthetic analogue--opilong]

Area of Science:

• Neuroscience
• Pharmacology
• Animal Behavior

Background:

• Opilong's effects on learning and central nervous system (CNS) function are not fully understood.
• Investigating the impact of repeated opilong administration on Wistar rat behavior and physiology is crucial.

Purpose of the Study:

• To evaluate the effects of repeated opilong injections (50 microg/kg/day) on the learning capabilities of Wistar rats.
• To assess the anxiolytic and analgesic properties of opilong.
• To investigate the impact of opilong on central nervous system excitability and blood coagulation.

Main Methods:

• Repeated administration of opilong (50 microg/kg/day) to Wistar rats.
• Assessment of learning performance under painful stimuli.
• Monitoring of central nervous system (CNS) excitability and psychoemotional reactions.
• Biochemical blood analysis to evaluate coagulation parameters.

Main Results:

• Opilong significantly enhanced learning (90% vs. 40% in controls) despite painful stimuli, indicating anxiolytic and analgesic effects.
• Opilong induced a shift towards excitation in the CNS, persisting even after the substance was eliminated.
• Increased peripheral sensitivity correlated with CNS hyper-excitability, manifesting as stress, neurotic reactions, and active avoidance.
• Biochemical analysis revealed prethrombotic conditions, including depressed fibrinolysis and hypercoagulation.

Conclusions:

• Opilong exhibits significant anxiolytic and analgesic effects, facilitating learning under stress.
• The substance induces a prolonged state of CNS hyper-excitability and altered psychoemotional responses.
• Opilong administration leads to prethrombotic changes in blood coagulation.
• Neuromediator brain systems are likely the primary targets of opilong, influencing CNS excitatory-inhibitory balance and conditioned responses.