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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Chemoenzymatic synthesis of two new halogenated coumarin glycosides as potential antifungal agents
Liangbin Zhou1, Ling Liu, Tian Tian
1College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
Abstract:
Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7-O-beta-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5-O-beta-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.
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