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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
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G-quadruplex DNA cleavage preference and identification of a perylene diimide G-quadruplex photocleavage agent using

Michelle Schoonover1, Sean M Kerwin

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA.

Bioorganic & Medicinal Chemistry
|November 20, 2012
PubMed

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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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