Related Experiment Video
Updated: May 16, 2026
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Multiplex bead suspension array for screening Neisseria gonorrhoeae antibiotic resistance genetic determinants in
Sergey Balashov1, Eli Mordechai, Martin E Adelson
1Femeris Women's Health Research Center, Medical Diagnostic Laboratories, LLC, a Member of the Genesis Biotechnology Group, Hamilton, New Jersey 08690, USA. sbalashov@mdlab.com
Abstract:
The increasing threat of antibiotic-resistant Neisseria gonorrhoeae highlights the need for new diagnostic options. A high-throughput multiplex bead suspension array assay was developed for profiling 29 N. gonorrhoeae genomic mutations and 2 plasmid genes conferring resistance to 6 antimicrobial agents: penicillin, ciprofloxacin, cefixime, tetracycline, azithromycin, and spectinomycin. The three steps of this assay include amplification of 12 N. gonorrhoeae chromosomal and plasmid loci, multiplex allele-specific primer extension reaction, and multiplex bead suspension array detection. Antibiotic resistance genetic determinants were identified successfully in 239 cervicovaginal N. gonorrhoeae-positive noncultured swab samples. This molecular assay can be used for detection of gonococci in clinical specimens, molecular typing, mutation profiling, and predictive assessment of N. gonorrhoeae susceptibility to antibiotics without the need for culture.
More Related Videos
08:04Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Published on: February 8, 2019
05:25Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Published on: July 14, 2023