Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment

A Gram-staining-negative, rod-shaped and non-motile bacterial strain, designated 12C25(T), was isolated from the crude-oil-degrading bacterial consortium enriched from mangrove sediment collected in Fujian Province, China. Optimal growth was observed at 25-28 °C, at pH 7.0 and in the presence of 2% (w/v) NaCl + 2% (w/v) KCl. Comparative 16S rRNA gene sequence analysis demonstrated that strain 12C25(T) shared the highest sequence similarity with members of the genus Muricauda (97.7-93.9%), exhibiting 97.7% sequence similarity and 33.7 ± 4% DNA-DNA relatedness to Muricauda aquimarina SW-63(T). The DNA G+C content of strain 12C25(T) was 39.9 mol%. The dominant fatty acids were iso-C15:1 G, iso-C17:0 3-OH, iso-C15:0, C18:0 and iso-C15:0 3-OH, and menaquinone with six isoprene units (MK-6) was the only respiratory quinone. On the basis of phenotypic data and phylogenetic inference, the novel strain belongs to the genus Muricauda, but can readily be distinguished from known species of this genus http://dx.doi.org/10.1601/nm.8170 and thus represents a novel species of the genus Muricauda. The name Muricauda zhangzhouensis sp. nov. is proposed and the type strain is 12C25(T) (=CGMCC 1.11028(T)=MCCC 1F01096(T)=DSM 25030(T)).