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Analysis of microRNA and gene expression profiling in triazole fungicide-treated HepG2 cell line
Yu Ri An1, Seung Jun Kim, Moon-Ju Oh
1Department of Biochemistry, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Republic of Korea.
Abstract:
MicroRNA (miRNA) plays an important role in various diseases and in cellular and molecular responses to toxicants. In the present study, we investigated differential expression of miRNAs in response to three triazole fungicides (myclobutanil, propiconazole, and triadimefon). The human hepatoma cell line (HepG2) was treated with the above triazoles for 3 h or 48 h. miRNA-based microarray experiments were carried out using the Agilent human miRNA v13 array. At early exposure (3h), six miRNAs were differentially expressed and at late exposure (48 h), three miRNAs were significantly expressed. Overall, this study provides an array of potential biomarkers for the above triazole fungicides. Furthermore, these miRNAs induced by triazoles could be the foundation for the development of a miRNA-based toxic biomarker library that can predict environmental toxicity.
Insights
This study identified specific microRNAs (miRNAs) that change in human liver cells exposed to common triazole fungicides. These findings suggest miRNAs could serve as biomarkers for fungicide toxicity.
Area of Science:
- Toxicology
- Molecular Biology
- Biochemistry
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression involved in disease and cellular responses to toxins.
- Triazole fungicides are widely used in agriculture, raising concerns about potential human health and environmental impacts.
Purpose of the Study:
- To investigate the differential expression of miRNAs in human hepatoma cells (HepG2) upon exposure to three triazole fungicides: myclobutanil, propiconazole, and triadimefon.
- To identify potential miRNA biomarkers for triazole fungicide exposure and toxicity.
Main Methods:
- Human hepatoma HepG2 cells were treated with myclobutanil, propiconazole, and triadimefon for 3 hours (early exposure) and 48 hours (late exposure).
- miRNA expression profiling was performed using Agilent human miRNA v13 arrays.
Main Results:
- Six miRNAs exhibited differential expression at early exposure (3h).
- Three miRNAs were significantly expressed at late exposure (48h).
Conclusions:
- The study identified specific miRNAs that are differentially expressed in response to triazole fungicides.
- These findings support the development of miRNA-based toxic biomarkers for predicting environmental toxicity and assessing triazole fungicide risks.
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