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[Differentiation of human sperm cells using flow cytometry]
F J Otto1, H Hettwer, N Hofmann
1Abteilung Experimentelle Tumorforschung, Fachklinik Hornheide, Münster.
Der Urologe. Ausg. A
|January 1, 1990
Abstract:
An improved method for differential staining and high-resolution flow cytometric measurement of human sperm cells is presented. Involving a mild pretreatment with citric acid/detergent and staining with DAPI, the new procedure provides excellent preservation and good discrimination of all cells present in normal and pathologic semen samples.