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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

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Published on: March 2, 2012

Recent progress in analytical capillary isotachophoresis.

Zdena Malá¹, Petr Gebauer, Petr Boček

¹Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.

Electrophoresis

|November 20, 2012

Summary

Analytical isotachophoresis (ITP) is crucial when combined with other methods, offering unique concentrating abilities for sensitive analyses and sample cleanup. Its applications in column switching, microchip techniques, and transient isotachophoresis are highlighted.

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Area of Science:

Analytical Chemistry
Separation Science
Electrophoresis

Background:

Analytical isotachophoresis (ITP) is a powerful separation technique.
While not frequently used alone, ITP excels in hyphenated systems.
Its inherent concentrating ability is key for high-sensitivity analysis.

Purpose of the Study:

To review the literature on analytical isotachophoresis (ITP) from 2010-2012.
To highlight the importance of ITP in combination with other analytical methods.
To showcase advancements in ITP theory, instrumentation, and applications.

Main Methods:

Literature review of analytical isotachophoresis (ITP) publications (2010-2012).
Analysis of theoretical studies, primarily computer simulations.
Examination of instrumentation, including column combinations and microchip techniques.
Categorization of applications based on hyphenated techniques (e.g., ITP-CZE, on-chip).

Main Results:

ITP's unique concentrating ability enables high sensitivity and sample cleanup.
Online combinations of ITP with other methods are of paramount importance.
Computer simulations significantly contribute to understanding ITP theory.
ITP demonstrates advantages in column switching and microchip formats.
Transient isotachophoresis offers benefits for detection and sampling.

Conclusions:

Analytical isotachophoresis, particularly in hyphenated systems, remains a vital technique for sensitive and efficient analyses.
Advancements in instrumentation and theoretical understanding continue to expand ITP's utility.
The concentrating power and sample enrichment capabilities of ITP are indispensable in modern analytical chemistry.