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Src kinase is a direct target of apigenin against UVB-induced skin inflammation

Sanguine Byun1, Jiman Park, Eunjung Lee

  • 1Department of Agricultural Biotechnology, WCU Biomodulation Major, Seoul National University, Seoul 151-921, Republic of Korea.

Carcinogenesis
|November 20, 2012
PubMed

Insights

Apigenin, a natural flavonoid, effectively reduces skin inflammation caused by UV radiation by inhibiting Src kinase activity. This study identifies Src as a key molecular target for apigenin's chemopreventive effects against UV damage.

Area of Science:

  • Biochemistry
  • Dermatology
  • Molecular Biology

Background:

  • Flavonoids, like apigenin found in fruits and vegetables, show potential anticarcinogenic properties.
  • The precise molecular targets and anti-inflammatory mechanisms of apigenin, particularly against UV-induced skin inflammation, require further elucidation.

Purpose of the Study:

  • To investigate the anti-inflammatory effects and molecular mechanisms of apigenin in UV-induced skin inflammation.
  • To identify the direct molecular target of apigenin in the context of UV radiation exposure.

Main Methods:

  • Utilized JB6 P+ cell lines and SKH-1 hairless mouse models to study UVB-induced inflammation.
  • Employed immunoblotting, kinase assays, and in vitro pull-down assays to analyze signaling pathways and molecular interactions.
  • Assessed UVB-induced ear edema, cyclooxygenase-2 (COX-2) expression, and Src kinase activity in vivo.

Main Results:

  • Apigenin inhibited UVB-induced cyclooxygenase-2 (COX-2) expression and restored upstream stimulatory factor levels.
  • Apigenin attenuated Src kinase activity, leading to the inhibition of UVB-induced phosphorylation of EGFR, MAPKs, and Akt signaling pathways.
  • In vivo studies confirmed that apigenin significantly reduced UVB-induced ear edema, COX-2 expression, and Src kinase activity.

Conclusions:

  • Apigenin demonstrates significant chemopreventive activity against UVB-induced skin inflammation.
  • The findings strongly suggest that apigenin targets Src kinase, thereby inhibiting key inflammatory signaling pathways and exerting its protective effects.

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