Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chiral Cages With Asymmetric π-Clefts Enable Catalytic Enantioconvergent S<sub>N</sub>1 Transformation via Synergistic Cation-π and Anion-π Interactions.

Angewandte Chemie (International ed. in English)·2026
Same author

Chiral Macrocycle-Enabled In-Situ Trapping of Catalytically Active Peroxometalate Anions for Directing Asymmetric Sulfoxidation.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Regulation of the Structure and Chiroptical Properties of Chiral Imidazolium Bismacrocycles via Specific Solvents.

Organic letters·2025
Same author

Synthesis, Structures, and CPL Property of Inner-Wall Modified Pagoda[5]Arenes Driven by Cavity Microenvironment.

Angewandte Chemie (International ed. in English)·2025
Same author

Effects of π-Extended Aromatic Fragments on Tris(hexa-<i>peri</i>-hexabenzocoronenyl)methyl Radical and Cation: Synthesis, Radical Transformation, and Reactivity Insights.

Organic letters·2025
Same author

Effects of combined preconception and prenatal myo-inositol, probiotics, and trace element supplementation on the outcomes of depressed mothers.

World journal of psychiatry·2025

Related Experiment Video

Updated: May 16, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

G4LDB: a database for discovering and studying G-quadruplex ligands.

Qian Li1, Jun-Feng Xiang, Qian-Fan Yang

  • 1Beijing National Laboratory for Molecular Sciences (BNLMS), Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.

Nucleic Acids Research
|November 20, 2012
PubMed
Summary

The G-quadruplex Ligands Database (G4LDB) compiles over 800 G-quadruplex ligands and associated data. This resource aids in discovering new drugs targeting G-quadruplexes for diseases like cancer.

More Related Videos

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 16, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Bioinformatics

Background:

  • G-quadruplexes are crucial DNA structures in telomeres and promoters, implicated in diseases like cancer.
  • Ligands targeting G-quadruplexes are vital for developing novel therapeutics.
  • A centralized resource for G-quadruplex ligands is needed to accelerate drug discovery.

Purpose of the Study:

  • To establish the G-quadruplex Ligands Database (G4LDB) for streamlining G-quadruplex ligand and drug discovery.
  • To compile comprehensive data on G-quadruplex ligand properties, structures, and activities.
  • To provide tools for G-quadruplex ligand design and facilitate the discovery of therapeutic agents.

Main Methods:

  • Collected and curated data on >800 G-quadruplex ligands and ~4000 activity records.
  • Developed a user-friendly web interface for data retrieval and analysis.
  • Integrated an online module for real-time prediction of ligand binding affinity.

Main Results:

  • G4LDB is the most extensive collection of G-quadruplex ligands currently available.
  • The database offers versatile search functionalities based on ligand properties, structures, and activities.
  • An integrated design module enables real-time prediction of ligand binding affinity.

Conclusions:

  • G4LDB serves as a valuable resource for researchers in G-quadruplex-targeted drug discovery.
  • The database facilitates the identification and design of novel therapeutic and diagnostic agents.
  • G4LDB supports advancements in understanding and treating G-quadruplex-related diseases.