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Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Cancer-Critical Genes I: Proto-oncogenes01:33

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Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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Published on: March 24, 2023

Metastasis-associated cell surface oncoproteomics.

Piia-Riitta Karhemo1, Maija Hyvönen, Pirjo Laakkonen

  • 1Research Programs Unit, Molecular Cancer Biology and Institute of Biomedicine, Biomedicum Helsinki, University of Helsinki Helsinki, Finland.

Frontiers in Pharmacology
|November 20, 2012
PubMed
Summary

Oncoproteomics identifies cell surface proteins crucial for cancer metastasis. This study reviews methods for analyzing these proteins, revealing potential new therapeutic targets.

Keywords:
biotinylationcancercell surfacemetastasisproteomics

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Area of Science:

  • Oncoproteomics
  • Cancer Biology
  • Proteomics

Background:

  • Cell surface proteins are vital in cancer and metastasis.
  • Hydrophobicity and low abundance limit their study in whole cell extracts.
  • Effective enrichment methods are needed to overcome these challenges.

Purpose of the Study:

  • To review proteomic analyses of metastasis-associated proteins.
  • To highlight challenges in solubilizing hydrophobic cell surface proteins.
  • To explore the identification of intracellular proteins on the cell surface.

Main Methods:

  • Cell surface biotinylation for protein enrichment.
  • Proteomic analysis of isolated cell surface proteins.
  • Review of existing literature on metastasis-associated proteins.

Main Results:

  • Identification of metastasis-associated proteins using cell surface biotinylation.
  • Challenges in solubilizing transmembrane proteins were confirmed.
  • Unexpected identification of intracellular proteins in cell surface samples.

Conclusions:

  • Cell surface proteomics, particularly using biotinylation, aids in discovering cancer markers and drug targets.
  • Further investigation is needed to understand the cell surface functions of identified intracellular proteins.
  • This approach enhances the understanding of cancer progression and metastasis.